• V. P. Barkar Engineering and Technological Institute “Biotechnica” Ukrainian Academy of Agrarian Sciences, Odesa, Ukraine
  • T. Yu. Markina G.S. Scovoroda Kharkiv National Pedagogical University, Kharkiv, Ukraine
Keywords: Heteroptera, Perillus bioculatus, artificial breeding, technocenosis, biomethod


This article presents an analysis of scientific literature on the ecological and biological features of the predatory bug Perillus bioculatus (Fabricius, 1775), a known entomophagous of Colorado potato beetle (Leptinotarsa decemlineata Say, 1824). The expediency of using this species as an agent of biological protection of plants, in particular potato plantations (Solanum tuberosum L, 1753) is considered. Domestic and European experience of stinkbug acclimatization in the 60-70s of the XX century is analyzed. In Ukraine, local populations of Perillus bioculatus are found in the Steppe and Forest-steppe zones, which indicates the ecological flexibility and adaptation of the species to local conditions, their favorable for the full development of insects. At this time, the biological features of P. bioculatus have been studied in sufficient detail, which is the basis for its economic use. The trophic specialization of the stinkbug is members of the family of Chrysomelidae, in particular the Colorado potato beetle. But in European countries there is a use of predators as food for other insect species. In parallel with the work on acclimatization in the second half of the last century in many countries began active research to determine the possibility of using Perillus bioculatus as an agent of biological plant protection. These studies are currently ongoing. Peculiarities of stinkbug breeding in artificial conditions, storage, methods of its introduction into agrocenoses are determined. Optimal modes of stinkbug retention have been developed. Air temperature + 25–27 0С, relative humidity 70-75%, photoperiod - 16 hours day / 8 hours night. According to the experience of domestic technical entomology, it is proposed to use the larvae of the mealworm beetle (Tenebrio molitor, Linnaeus, 1758) and the greater wax moth (Galleria mellonella Linnaeus, 1758) to feed the stinkbug under artificial conditions. The prospects of making artificial nutrient mixtures are noted. There is no need for sophisticated equipment to breed the stinkbug. It is quite suitable available, which is used to develop other entomophagous. For the needs of the biomethod, the most appropriate use of P. bioculatus in the preimaginal stage, as adult insects are able to leave the place of release, especially at high densities. It is shown that there is no effective technology of mass breeding and use of this species to this day.


Adams, T.S., 2000. Effect of Diet and Mating on Oviposition in the Twospotted Stink Bug Perillus bioculatus (F.) (Heteroptera: Pentatomidae). Annals of the Entomological Society of America, 93 (6): 1288–1293. DOI: 10.1603/0013-8746(2000)093.

Agasieva, I.S., Ismailov,V.Y., Nefedova, M.V. & Fedorenko, E.V., 2016. Development of elements biocenotic regulation main pests of solanaceous crops. Issue 9. Proceedings of the International Conference“Biological Plant Protection as the Basis of Ecosystem Stabilization”with Strategic Youth Session “Manpower, Resources, Opportunities, Innovations”: (20-22 September 2016. Krasnodar): 103–106. (in Russian: Агасьева, И.С., Исмаилов, В.Я., Нефедова, М.В., Федоренко Е.В. Разработка элементов биоценотической регуляции численности основных вредителей картофеля).

Agasieva, I.S., Nefedova,M.V., Fedorenko,E.V. & Umarova, A.O., 2017. Biological features of pentatomide predaceous bugs and new methods of their cultivation. International research journal, 7 (61): 6–8. (in Russian: Агасьева, И.С. , Нефедова, М.В. , Федоренко, Е.В. , Умарова, А.О. Биологические особенности хищных клопов пентатомид и новые методы их культивирования на ипс.). DOI: 10.23670/IRJ.2017.61.079.

Almady, S., Khelifi, M. & Beaudoin, M.P., 2012. Control of the Colorado Potato Beetle, Leptinotarsa decemlineata (Say), Using Predator Insects Released by a Mechanical Prototype. Journal of Environmental Science and Engineering. 1(11): 1279-1287. [online] Available at: <> [Accessed 20 November 2012].

Alyokhin, A., Dively, G., Patterson, M., Castaldo, C., Rogers, D., Mahoney, M. & Wollam, J., 2007. Resistance and cross-resistance to imidacloprid and thiamethoxam in the Colorado potato beetle Leptinotarsa decemlineata. Pest Management Science, 63(1): 32-41. DOI: 10.1002/ps.1305.

Biever, K.D. & Chauvin, R.l., 1992. Suppression of the Colorado Potato Beetle (Coleoptera:Chrysomelidae) with Augmentative Releases of Predaceous Stinkbugs (Hemiptera:Pentatomidae). Journal of Economic Entomology, 85(3): 720-726. DOI: 10.1093/jee/85.3.720.

Bhusal, D.R., Patel P., Ghimire, K.C., Bista, M. & Kumar, B., 2019. Size-based intraspecific variations along altitudinal gradient and follow of Bergmann’s rule in Parthenium beetle, Zygogramma bicolorata Pallister. Journal of Asia-Pacific Entomology, 22(4): 1173-1179. [online] Available at: [Accessed 17 October 2019]. DOI: 10.1016/j.aspen.2019.10.008.

Cingel, A., Savić, J., Lazarević, J., Cosić, T., Raspor, M., Smigocki, A. & Ninković, S., 2016. Extraordinary Adaptive Plasticity of Colorado Potato Beetle: “Ten-Striped Spearman” in the Era of Biotechnological Warfare. International Journal of Molecular Sciences. [online] Available at: <>. [Accessed 5 September 2016]. DOI: 10.3390/ijms17091538.

Cloutier, C. & Bauduin, F., 1995. Biological control of the Colorado potato beetle Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) in Quebec by augmentative releases of the two-spotted stinkbug Perillus bioculatus (Hemiptera: Pentatomidae). The Canadian Entomologist, 127: 195-212. DOI: 10.4039/Ent127195-2.

Coudron, T. A. & Kim, Y., 2004. Life History and Cost Analysis for Continuous Rearing of Perillus bioculatus (Heteroptera: Pentatomidae) on zoophytogenous Artificial Diet. Journal of Economic Entomology, 97(3): 807-812. DOI: 10.1603/0022-0493(2004)097[0807:lhacaf];2.

Coudron, T.A., Popham, H. J.R. & Ellersieck, M.R., 2009. Influence of diet on cold storage of the predator Perillus bioculatus (F.). BioControl , 54 (6): 773–783. DOI: 10.1007 / s10526-009-9227-8.

De Clercq, P., 2008. In: Capinera, J.L ed. Encyclopedia of Entomology. 2008. Two-Spotted Stink Bug, Perillus bioculatus (Fabricius) (Hemiptera: Pentatomidae, Asopinae): 4004–4006.

De Ladurantaye, Y., Khelifi, M.,.Cloutier, C. & Coudron, T.A., 2010. Short-term storage conditions for transport and farm delivery of the stink bug Perillus bioculatus for the biological control of the Colorado potato beetle. Canadian Biosystems Engineering, 52: 4.1-4.5. [online] Available at: <> [Accessed 20 July 2010]. Identifier: C0814

Derzhavna sluzhba statystyky Ukrainy. Rehionalna statystyka. Silske, lisove ta rybne hospodarstvo. Roslynnytstvo. [online] Available at: <> (in Ukrainian: Державна служба статистики України)

Duursun, A. & Fent, M., 2018. Erst nach weis von Perillus bioculatus (Fabricius, 1775) (Hemiptera: Heteroptera: Pentatomidae) für Anatolien (Türkei). In: Mitteilungsblatt der Arbeitsgruppe Mitteleuropäischer Heteropterologen. 53, Köln, Deutchland: 18–20.

Elisoveczkaya, D.S., Derzhanskij, V.V. & Doroshenko, V.N., 2015. Perillus bioculatus in Moldova: the

problems of preservation of the population and the use of predator to protect potatoes against Colorado potato beetle. In: Materials of the III International Conference, dedicated to the 110 th anniversary of the birth of Academician N.V. Smolsky. “Biodiversity conservation problems and use of biological resources”. Minsk, Belarus, 7–9 October: 116–118. (in Russian: Елисовецкая, Д.С., Держанский, В.В., Дорошенко, В.Н. Perillus bioculatus в Молдове: проблемы сохранения популяции и возможность использования хищника для защиты картофеля от колорадского жука).

Franz, J., 1957. Beobachtungen uber die naturliche Sterblichkeitdes Kartoffelkafers (Leptinotarsa decemlineata, Say) in Kanada. Entomophaga, 2: 197–212.

Gusev, G.V., 1991. Entomophages of the Colorado potato beetle. Moscow: Agropromizdat (in Russian: Гусев, Г.В. Энтомофаги колорадского жука).

Hough-Goldstein, J., Janis, J.A. & Ellers, C.D., 1996. Release Methods for Perillus bioculatus (F.), a Predator of the Colorado Potato Beetle. Biological control, 6(1):114–122 DOI: 10.1006/bcon.1996.0014.

Ismailov, V.I., Agasieva, I.S., Kiel, V.I., Fedorenko, E.V. & Besedina, E.N., 2014. The Studies of Biological Characteristics and Morphogenetic Structure of Predatory Stinkbug PerilliusBioculatus. Agro XXI, 4-6: 26–28. (in Russian: Исмаилов, В.Я., Агасьева, И.С., Киль, В.И., Федоренко, Е.В., Беседина, Е.Н. Биологические особенности и результаты морфогенетического анализа хищного клопа perillus bioculatus).

Ismailov, V.Ya., Agas’eva, I.S., Kil, V.I., Fedorenko, E.V., Nefedova, M.V. & Besedina, E.N., 2019. Biological Characterization, Phenotypic and Genotypic Structure of Predatory Stinkbug Perillius Bioculatus Fabr. (Heteroptera, Pentatomidae) Population in Krasnodar Region. Sel`skokhozyaistvennaya biologiya, 54(1): 110-120. (in Russian: Исмаилов, В.Я., Агасьева, И.С., Киль, В.И., Федоренко, Е.В., Беседина, Е.Н. Биология, фенотипическая и генотипическая структура популяций хищного клопа Perillus bioculatus Fabr. (Heteroptera, Pentatomidae) в Краснодарском крае). doi: 10.15389

Jermy, T., 2008. The introduction of Perillus bioculatus into Europa to control the Colorado beetle. EPPO Bulletin, 10 (4): 475-479. DOI: 10.1111/j.1365-2338.1980.tb01733.x

Kivan, M., 2004. Some observations on Perillus bioculatus (F.) (Heteroptera: Pentatomidae) a new record for the entomofauna of Turkey. Turkish Journal of Entomology, 28(2): 95–98.

Lachance, S. & Cloutier, C., 1997. Factors Affecting Dispersal of Perillus bioculatus (Hemiptera: Pentatomidae), a Predator of the Colorado Potato Beetle (Coleoptera: Chrysomelidae). Environmental entomology, 26(4): 946–954. DOI: 10.1093/ee/26.4.946.

Lenteren, J., 2012. The state of commercial augmentative biological control: Plenty of natural enemies, but a frustrating lack of uptake. BioControl, 57(1): 1–20. DOI: 10.1007/s10526-011-9395-1.

Levchenko, I.S. & Martynov, V.V., 2018. New and interesting records of stink bugs (Heteroptera: Pentatomoidea) in Donbass. Caucasian Entomological Bulletin, 14(2): 141–145. (in Russian: Левченко, И.С., Мартынов В.В. Новые и интересные находки клопов-щитников (Heteroptera: Pentatomoidea) в Донбассе.) DOI: 10.23885/181433262018142-141145.

Loon, J.J.A, De Vos, E.W. & Dicke, M., 2000. Orientation behavior of the predatory hemipteran Perillus bioculatus to plant and prey odours. Entomologia Experimentalis et Applicata, 96(1): 51–58. DOI: 10.1046/j.1570-7458.2000.00678.x.

Matlock, R. B., 2005. Impact of Prey Size on Prey Capture Success, Development Rate, and Survivorship in Perillus bioculatus (Heteroptera: Pentatomidae), a Predator of the Colorado Potato Beetle. Environmental entomology, 34(5): 1048–1056. DOI: 10.1603/0046-225X(2005)034[1048:IOPSOP]2.0.CO;2

Markina, T.Yu., Putchkov, O.V. & Fedyay, I.O., 2018. New and little-known species of the bugs (Insecta, Heteroptera) of fauna of Ukraine. Biologiya ta valeologiya, 20: 43–48. (in Ukrainian: Маркіна, Т.Ю., Пучков, О.В. та Федяй, І.О. Нові та маловідомі види клопів (Insecta: Hemiptera, Heteroptera) для фауни України) DOI: 10.5281/zenodo.2543598

Tamaki, G. & Butt, B. A., 1978. A source of information. In: Technical Bulletin, 1581. 1978. Impact of Perillus Bioculatus on the Colorado Potato Beetle and Plant Damage. U.S. Department of Agriculture.

Rabitsch, W., 2010. True bugs (Hemiptera, Heteroptera). BioRisk, 4(1): 407–433. DOI: 10.3897/biorisk.4.44

Rathee, M. & Ram, P., 2018. Impact of cold storage on the performance of entomophagous insects: an overview. Phytoparasitica, 46: 421–449. [online] Available at: <>.[Accessed 13 July 2018]. DOI: 10.1007/s12600-018-0683-5

Rinkevich, F.D., Cathy, Su., Lazo, T.A., Hawthorne, D.J., Tingey, W.M., Naimov, S. & Scott, J.G., 2012. Multiple evolutionary origins of knockdown resistance (kdr) in pyrethroid-resistant Colorado potato beetle, Leptinotarsa decemlineata. Pesticide Biochemistry and Physiology, 104(3): 192–200. DOI: 10.1016/j.pestbp.2012.08.001

Rojas, M.G., Morales-Ramos, J.A. & King, E.G., 2000. Two Meridic Diets for Perillus bioculatus (Heteroptera: Pentatomidae), a Predator of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Biological Control, 17: 92–99. [online] Available at: <> [Accessed 12 August 1999]. DOI: 10.1006/bcon.1999.0780

Saint-Cyr, J.F. & Cloutier, C., 1996. Prey Preference by the Stinkbug Perillus bioculatus, a Predator of the Colorado Potato Beetle. Biological Control, 7(3): 251–258. DOI: 10.1006/bcon.1996.0091.

Tarla, S. & Tarla, G., 2018. Detection of Perillus bioculatus (F.) (Heteroplera: Pentatomidae) on a New Host in Anatolia. International Journal of Agriculture Innovations and Research, 7(3): 317-319. [online] Available at: [Accessed 20 December 2018].

Velyka ukrainska entsyklopediia. Silskohospodarski nauky. Ahronomiia. Ahroklimatychne raionuvannia. [online] Available at: <> (in Ukrainian: Велика українська енциклопедія).

Wegorek, P., 2002. Insecticide resistance management strategy for Colorado potato beetle (Leptinotarsa decemlineata Say) in Poland. Resistant Pest Management Newsletter, 11(2): 22-30.

Yocum, G.D., Coudron, T.A. & Brandt, S.L., 2006. Differential gene expression in Perillus bioculatus nymphs fed a suboptimal artificial diet. Journal of Insect Physiology, 52: 586-592. [online] Available at: <> [Accessed 13 February 2006]. DOI: 10.1016/j.jinsphys.2006.02.00.